Thermo Fisher Scientific CD20 Monoclonal Antibody (L26)
CD20 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간 시료에 반응하며, Protein G로 정제된 액상 형태. PBS 완충액에 보관하며 4°C에서 안정적으로 저장 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:250 |
| Flow Cytometry (Flow) | 0.3 µL / 1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L26 |
| Immunogen | Human tonsil B cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.30 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, as well as a subset of T lymphocytes and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts, and its expression is synchronous with surface IgM. It remains on the cell surface after monoclonal antibody treatment, making it a useful target for antibody-mediated B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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