Thermo Fisher Scientific p53 Monoclonal Antibody (DO-1)
인간 p53 단백질을 인식하는 DO-1 클론 단일클론 항체. Western blot, IHC, Flow cytometry 등 다양한 응용에 적합. 단백질 G로 정제된 액상 형태로 제공되며, 4°C에서 보관. 연구용으로만 사용 가능.
- 카탈로그번호
- A500015Ax (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific p53 Monoclonal Antibody (DO-1)
Applications and Tested Dilution
| Application | Tested Dilution / Usage |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10⁶ cells |
| Immunoprecipitation (IP) | 20 µL per 1 mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DO-1 |
| Immunogen | Recombinant full-length human p53 (N-terminal, epitope within aa 20–25) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Protein G |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 activation induces expression of genes such as p21waf1, regulating G1 or G2/M arrest.
Structurally, p53 includes:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerization domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is critical for activation and stabilization. Phosphorylation at Ser392 correlates with tumor formation. p53 plays a key role in preventing proliferation of damaged cells and acts as a tumor suppressor. Mutations in TP53 disrupt DNA binding and tumor suppression, contributing to cancer development. Germline TP53 mutations are associated with Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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