Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), Super Bright 600, eBioscience
인간 CD42b(platelet gpIb)를 인식하는 HIP1 단일클론 항체로, Super Bright 600 형광체 결합 제품입니다. 유세포 분석용으로 최적화되어 있으며, 405 nm 레이저에 의해 여기되어 600 nm에서 방출됩니다. 고감도 분석에 적합하며 광에 민감하므로 어둡게 보관해야 합니다.
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Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), Super Bright 600, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIP1 |
| Conjugate | Super Bright™ 600 |
| Excitation/Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762541 |
Additional Formats
- Unconjugated
- APC
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Yellow 610
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 702
- Request custom conjugation
Product Specific Information
Description:
The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.
Applications Reported:
The HIP1 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and validated for flow cytometric analysis of human platelets. Recommended usage: 5 µL (0.5 µg) per test in a final volume of 100 µL. Suitable cell number range: 10⁵–10⁸ cells/test.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the 405 nm violet laser and emits at 600 nm. Recommended detection filter: 610/20 bandpass.
Usage Notes:
- When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
- Light sensitivity: Protect vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 2–8°C in the dark.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD42b is a platelet activation marker involved in coagulation and aggregation. It interacts with extracellular matrix and adhesion molecules, forming part of the GPIb-IX-V receptor complex, which is essential for platelet tethering to thrombogenic surfaces. CD42b binds von Willebrand factor (VWF) and other coagulation factors, contributing to thrombus formation and metastasis. It is expressed on platelets and megakaryocytes. Mutations in CD42b are associated with Bernard-Soulier syndrome and platelet-type von Willebrand disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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