Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), Super Bright 600, eBioscience

Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), Super Bright 600, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 645
• Request custom conjugation

Product Specific Information

The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). Initially known to be expressed on platelets and megakaryocytes, CD41 is also found on hematopoietic progenitors in embryos, fetuses, and adults. CD41 forms a receptor with CD61 (gpIIIa, integrin beta III) that mediates platelet adhesion through fibrinogen, fibronectin, and vitronectin binding.

CD150+CD48−CD41− expression efficiently identifies hematopoietic stem cells (HSCs) with enhanced reconstitution potential.
Reported application: flow cytometric analysis.
Tested application: flow cytometric analysis of mouse platelets using ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize antibody titration per assay.

Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm; recommended filter: 610/20 bandpass.
Use Super Bright Complete Staining Buffer (Product # SB-4401) when staining with multiple Super Bright conjugates to minimize polymer interactions.
Light sensitive—protect from exposure.
Samples may be fixed using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) consists of two subunits (120 kDa alpha and 23 kDa beta) that interact with CD61 in the presence of calcium to form a functional adhesive receptor. CD41 mediates platelet aggregation and binds von Willebrand factor, fibrinogen, fibronectin, and vitronectin during vessel injury. Expressed mainly in megakaryocyte-platelet lineages and early hematopoietic differentiation stages.
Associated diseases include Glansmann Thrombasthenia and Platelet-type 16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures.

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