Thermo Fisher Scientific GTF3C3/TFIIIC102 Polyclonal Antibody
Rabbit polyclonal antibody targeting human GTF3C3/TFIIIC102, validated for WB, IHC, and IP applications. High specificity and affinity-purified for reliable detection. Suitable for research use in transcription factor and RNA polymerase III studies.
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- A301238AT
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Thermo Fisher Scientific GTF3C3/TFIIIC102 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 475 and 525 of human general transcription factor 3C polypeptide 3 (Transcription factor IIIC 102 kDa subunit) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
RNA polymerase III synthesizes tRNA, 5s rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. To maintain rapid growth, cells require high levels of Pol III-transcribed RNA, dependent on TFIIIB and TFIIIC2 transcription factor complexes.
The TFIIIC2 complex consists of TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, and TFIIIC63 subunits, which are overexpressed in malignant cells such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may play a key role in tumor cell deregulation.
TFIIIB includes TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1), and TFIIIB, all upregulated in transformed cells. In many carcinomas, tumor suppressors RB and p53 are inactivated, impairing their ability to bind and suppress TFIIIB function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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