Thermo Fisher Scientific IFT57 Monoclonal Antibody (OTI5B6), TrueMAB
Human IFT57 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB 및 IHC(P) 실험에 적합. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 세포섬모 형성 및 hedgehog 신호전달 관련 연구에 유용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5B6 |
| Immunogen | Full-length human recombinant IFT57 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
HIPPI is required for the formation of cilia and plays an indirect role in sonic hedgehog signaling. It has pro-apoptotic function through interaction with HIP1, recruiting caspase-8 (CASP8) and triggering apoptosis.
HIPPI can bind to the DNA sequence motif 5'-AAAGACATG-3' found in the promoters of caspase genes (CASP1, CASP8, CASP10), suggesting a possible transcription regulatory role, although its relevance remains unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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