Thermo Fisher Scientific IFT57 Monoclonal Antibody (OTI1H2), TrueMAB
Human IFT57 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB 및 ICC/IF에 사용 가능. Lyophilized 형태로 제공되며, PBS buffer 및 trehalose 함유. 세포 내 섬모 형성과 apoptosis 관련 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H2 |
| Immunogen | Full length human recombinant protein of human IFT57 (NP_060480) produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
HIPPI is required for the formation of cilia and plays an indirect role in sonic hedgehog signaling. It has pro-apoptotic function via interaction with HIP1, recruiting caspase-8 (CASP8) and triggering apoptosis.
HIPPI can bind the DNA sequence motif 5'-AAAGACATG-3' found in promoters of caspase genes such as CASP1, CASP8, and CASP10, suggesting a possible transcription regulatory role, though its relevance remains unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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