Thermo Fisher Scientific Goat anti-Rabbit IgG (Heavy chain) Superclonal Secondary Antibody, Alexa Fluor Plus 647
Alexa Fluor Plus 647로 결합된 염소 유래 항-토끼 IgG(Heavy chain) 2차 항체로, WB, ICC/IF, Flow Cytometry에 적합. 높은 특이성과 로트 간 일관성 제공. 낮은 교차 반응성과 밝은 형광 신호로 정량 및 영상 분석에 최적화.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–4 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit Immunoglobulin |
| Conjugate | Alexa Fluor™ Plus 647 |
| Excitation / Emission Max | 658 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Wet ice |
| RRID | AB_2921063 |
| Target | IgG (Heavy chain) |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Biotin
- HRP
- Custom conjugation available upon request
Product Specific Information
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and superior lot-to-lot consistency.
Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed.
Alexa Fluor Plus secondary antibodies offer brighter signals and improved signal-to-noise ratios compared to conventional Alexa Fluor conjugates. They utilize proprietary dye chemistry for enhanced fluorescent performance, enabling clearer imaging and better detection of low-abundance targets.
Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to avoid aggregates that may cause nonspecific background staining. Optimal antibody dilution should be determined empirically for each application.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified reagents with well-characterized specificity for rabbit immunoglobulins. They are used in detection, sorting, or purification of rabbit IgG and enable multiple detection systems (e.g., HRP, AP, fluorescence). These antibodies provide signal amplification as multiple secondary antibodies can bind to a single primary antibody. Typically, they are generated by immunizing the host with pooled rabbit immunoglobulins and further purified or modified for high specificity and performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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