
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor Plus 405
Alexa Fluor Plus 405 형광을 결합한 염소 유래 항-마우스 IgG(H+L) Superclonal 2차 항체로, ICC/IF 및 Flow Cytometry에 최적화되어 있습니다. 재조합 항체 기술로 일관된 품질과 우수한 특이성을 제공하며, 밝은 형광 신호를 통해 고해상도 이미징에 적합합니다.
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- A55057
- 카테고리
- Antibodies
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor Plus 405
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–8 µg/mL |
| Flow Cytometry (Flow) | 0.5–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse Immunoglobulin |
| Conjugate | Alexa Fluor™ Plus 405 |
| Excitation/Emission Max | 404 / 455 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Wet ice |
| RRID | AB_2921065 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and superior lot-to-lot consistency.
Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed.
Obtain superior images with the new Invitrogen Alexa Fluor Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, offering better signal-to-noise for critical experiments. These antibodies use proprietary dye chemistry to generate stunning fluorescent images.
Usage Tip:
Centrifuge the conjugate solution briefly before use and add only the supernatant to your experiment. This helps remove protein aggregates and reduces nonspecific background staining. Optimal dilution should be determined empirically.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of their targets. Secondary antibodies increase versatility by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
Most secondary antibodies are generated by immunizing the host with pooled immunoglobulins from the target species and can be purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Formats
- Agarose
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- Biotin
- DyLight series (350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG)
- FITC
- HRP
- PE
- Rhodamine
- TRITC
- WesternDot 625
- Custom conjugation available upon request
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