Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor Plus 405

Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor Plus 405

Applications and Tested Dilution

Product Specifications

Product Specific Information

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and superior lot-to-lot consistency.

Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed.

Obtain superior images with the new Invitrogen Alexa Fluor Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, offering better signal-to-noise for critical experiments. These antibodies use proprietary dye chemistry to generate stunning fluorescent images.

Usage Tip:
Centrifuge the conjugate solution briefly before use and add only the supernatant to your experiment. This helps remove protein aggregates and reduces nonspecific background staining. Optimal dilution should be determined empirically.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of their targets. Secondary antibodies increase versatility by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification.

Most secondary antibodies are generated by immunizing the host with pooled immunoglobulins from the target species and can be purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Available Formats

• Agarose
• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Alexa Fluor 680
• Alexa Fluor 790
• Alexa Fluor Plus 488
• Alexa Fluor Plus 647
• AP
• Biotin
• DyLight series (350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG)
• FITC
• HRP
• PE
• Rhodamine
• TRITC
• WesternDot 625
• Custom conjugation available upon request

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