
Thermo Fisher Scientific Goat anti-Rabbit IgG (Heavy chain) Superclonal Secondary Antibody, Alexa Fluor Plus 488
Alexa Fluor Plus 488 형광을 결합한 염소 유래 항-토끼 IgG (Heavy chain) 2차 항체로, WB, ICC/IF, Flow Cytometry에 최적화. 재조합 Superclonal 기술로 높은 특이성과 로트 간 일관성 제공. 다양한 형광 및 효소 결합 포맷 지원. 연구용 전용.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (Heavy chain) Superclonal Secondary Antibody, Alexa Fluor Plus 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–4 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit Immunoglobulin |
| Conjugate | Alexa Fluor™ Plus 488 |
| Excitation / Emission Max | 493 / 518 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2921061 |
| Target | IgG (Heavy chain) |
| Antibody Form | Whole Antibody |
Available Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 647
- Biotin
- HRP
- Custom conjugation available upon request
Product Specific Information
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and superior lot-to-lot consistency.
Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed.
Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, offering better signal-to-noise ratio for critical experiments. These antibodies use proprietary dye chemistry for enhanced fluorescence and image quality.
Usage Tip:
Centrifuge the conjugate solution briefly before use and use only the supernatant to minimize nonspecific background staining. Optimal dilution should be determined empirically for each application.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit targets. Secondary antibodies increase versatility and sensitivity through signal amplification and can be modified via immunoaffinity purification, fragmentation, or conjugation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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