Thermo Fisher Scientific CD181 (CXCR1) Monoclonal Antibody (eBio8F1-1-4 (8F1-1-4)), PE, eBioscience

Thermo Fisher Scientific CD181 (CXCR1) Monoclonal Antibody (eBio8F1-1-4 (8F1-1-4)), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 1 publication

Product Specifications

Product Specific Information

The eBio8F1-1-4 (8F1-1-4) monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha).
CD181 is a 67–70 kDa member of the 7-transmembrane G-protein coupled receptor (GPCR) family.
It is expressed as a homodimer or heterodimer with CD182 (CXCR2, IL-8Rbeta) on granulocytes, NK cells, T cell subsets, mast cells, monocytes, endothelial cells, megakaryocytes, and oligodendrocytes.

Ligands such as IL-8, NAP-2, GCP-2, and GRO-alpha induce cell activation, chemotaxis, proliferation, and angiogenesis.
CD181 mediates phospholipase D activation and respiratory burst, while CD182 does not.
IL-8 primarily mediates neutrophil function via CD181.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test (100 µL total volume).
Cell count range: 10^5–10^8 cells/test.

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green

Target Information

CXCR1 is a G-protein-coupled receptor for interleukin 8 (IL8).
It binds IL8 with high affinity and transduces signals via G-protein activated second messenger systems.
Mouse knockout studies suggest CXCR1 inhibits embryonic oligodendrocyte precursor migration in the developing spinal cord.
The IL8RB gene, encoding another high-affinity IL8 receptor, and IL8RBP pseudogene form a gene cluster on chromosome 2q33–q36.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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