Thermo Fisher Scientific CD182 (CXCR2) Monoclonal Antibody (eBio5E8-C7-F10 (5E8-C7-F10)), PE, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test
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Product Specifications

Additional Formats

• Unconjugated
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8R beta), a 67–70 kDa seven-transmembrane GPCR family member.
CD182 forms homodimers or heterodimers with CD181 (CXCR1) and is expressed on granulocytes, NK cells, subsets of T cells, mast cells, monocytes, endothelial cells, megakaryocytes, and oligodendrocytes.

Ligands include IL-8, NAP-2, GCP-2, GROα, GROβ, GROγ, which induce cell activation, chemotaxis, proliferation, and angiogenesis.
While both CD181 and CD182 mediate chemotaxis and calcium signaling, only CD181 induces phospholipase D activation and respiratory burst. IL-8 primarily mediates neutrophil function through CD181.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry on normal human peripheral blood at 5 µL/test (0.25 µg/test).
A test is defined as the amount of antibody sufficient to stain a sample in 100 µL final volume (10⁵–10⁸ cells/test).

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CXCR2 is a receptor for interleukin-8, a potent neutrophil chemotactic factor and member of the GPCR family.
Binding of IL-8 activates neutrophils via a G-protein-mediated phosphatidylinositol-calcium second messenger system.
CXCR2 binds IL-8, GRO/MGSA, and NAP-2 with high affinity and is expressed across various tissues.
ESTs have been isolated from human placenta and thymus libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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