Thermo Fisher Scientific Vinculin Polyclonal Antibody
Rabbit polyclonal antibody targeting human Vinculin (VCL), validated for WB, IHC, and ICC/IF. Recommended antigen retrieval using citrate buffer. Key cytoskeletal protein linking actin to membranes. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human vinculin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2550102 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
Suggested positive control tissues: pancreas or small intestine.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions.
It functions as one of several interacting proteins involved in anchoring F-actin to the membrane.
In adhesion plaque assembly, the beta subunit of integrin binds to talin, which binds to vinculin that interacts with alpha-actinin and possibly with itself.
Since alpha-actinin binds and cross-links actin filaments, vinculin is a key element in linking the extracellular matrix to the cytoplasmic microfilament system.
Mutations in VCL can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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