Thermo Fisher Scientific ISG15 Recombinant Rabbit Monoclonal Antibody (2C6V1)
ISG15 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 래빗 모노클로날 항체. Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG 형식의 액상 항체.
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Thermo Fisher Scientific ISG15 Recombinant Rabbit Monoclonal Antibody (2C6V1)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2C6V1 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–165 of human ISG15 (NP_005092.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3093760 |
Product Specific Information
Immunogen sequence:
MGWDLTVKML AGNEFQVSLS SSMSVSELKA QITQKIGVHA FQQRLAVHPS GVALQDRVPL ASQGLGPGST VLLVVDKCDE PLSILVRNNK GRSSTYEVRL TQTVAHLKQQ VSGLEGVQDD LFWLTFEGKP LEDQLPLGEY GLKPLSTVFM NLRLRGGGTE PGGRS
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and augmentation of non-MHC-restricted cytotoxicity.
ISG15 synthesis is stimulated by IFN-alpha, IFN-beta, or IFN-omega, but not IFN-gamma. Its expression is induced by overexpression of interferon regulatory factors and by influenza B virus.
ISG15 is secreted by monocyte, T-lymphocyte, B-lymphocyte, fibroblast, and epithelial cell lines. It functions as a member of the cytokine cascade, amplifying immunomodulatory effects of interferons, and also acts intracellularly as a ubiquitin homolog.
Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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