Thermo Fisher Scientific EIF4A1/EIF4A2/EIF4A3 Recombinant Rabbit Monoclonal Antibody (10P2J5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000-1:80,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:4,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-600 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

10P2J5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4A1/EIF4A2/EIF4A3 (NP_001407.1/NP_001958.2/NP_055555.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EIF4A1/EIF4A2/EIF4A3,uniProtId:P38919-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:411,antigenImageFileName:MA5-51538_EIF4A1EIF4A2EIF4A3_P38919-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51538_EIF4A1EIF4A2EIF4A3_P38919-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:EIF4A1/EIF4A2/EIF4A3,uniProtId:P60842-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:406,antigenImageFileName:MA5-51538_EIF4A1EIF4A2EIF4A3_P60842-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51538_EIF4A1EIF4A2EIF4A3_P60842-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:EIF4A1/EIF4A2/EIF4A3,uniProtId:Q14240-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:407,antigenImageFileName:MA5-51538_EIF4A1EIF4A2EIF4A3_Q14240-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51538_EIF4A1EIF4A2EIF4A3_Q14240-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3094412

Product Specific Information

Immunogen sequence: MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA

Target Information

The eIF4A proteins are involved in many aspects of RNA metabolism due to their RNA-binding capacity and ATPase activity. eIF4A1 and eIF4A2 are cytoplasmic, whereas eIF4A3 is nuclear-localized. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5`-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.