Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (RMT3-23), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.5 µg/test
• Publications: View 13 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Brilliant Violet 421
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 702
• Request custom conjugation

Product Specific Information

The RMT3-23 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. It recognizes CD366 expressed in both BALB/c and C57BL/6 mouse strains. CD366 is a type I transmembrane protein containing an immunoglobulin and mucin-like domain extracellularly, and a tyrosine phosphorylation motif intracellularly.

CD366 is selectively expressed by differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells, but absent on Th2/Tc2 cells and other hematopoietic cell types such as naïve T cells, B cells, macrophages, and dendritic cells. Expression increases at late stages of Th1 differentiation, suggesting roles in effector function rather than differentiation.

In experimental autoimmune encephalomyelitis (EAE), CD366 is expressed on most CD4⁺ and CD8⁺ T cells in the CNS at disease onset, while peripheral CD4⁺ cells show minimal expression.

Functional activity: RMT3-23 blocks DC recognition of apoptotic cells and induces autoantibody production.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration per assay.

Fluorochrome information:

• Excitation: 405 nm
• Emission: 436 nm
• Recommended filter: 450/50 bandpass
• Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright conjugates.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

TIM3 (HAVCR2) is a 281-amino acid, type I Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells. It includes an IgV-like and mucin-like extracellular domain, and a conserved tyrosine phosphorylation motif intracellularly. TIM3 is involved in macrophage activation, autoimmune disease induction, and regulation of Th1-mediated immune responses, promoting immune tolerance. It has pathological relevance in EAE (Experimental Autoimmune Encephalomyelitis) and can enhance disease severity in mice.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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