Thermo Fisher Scientific Phospho-p47phox (Ser359) Polyclonal Antibody
Phospho-p47phox (Ser359)에 특이적인 Rabbit Polyclonal Antibody로, 인산화된 NCF1/p47-phox 단백질 검출에 사용됩니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 고순도 및 안정적인 PBS/glycerol buffer로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NCF1 (Accession P14598), corresponding to amino acid residues around phosphorylated Ser359. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818292 |
Product Specific Information
Antibody detects endogenous levels of NCF1/p47-phox only when phosphorylated at Serine 359.
Target Information
p47phox (NCF1) is required, along with NCF2 and membrane-bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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