Thermo Fisher Scientific DUT Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 0.5–1 µg/mL

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate.
This reaction serves two cellular purposes:

1. Providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication.
2. Limiting intracellular pools of dUTP.

Elevated levels of dUTP lead to increased incorporation of uracil into DNA, inducing extensive excision repair mediated by uracil glycosylase.
This repair process, resulting in the removal and reincorporation of dUTP, leads to DNA fragmentation and cell death.
Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus.
A related pseudogene is located on chromosome 19.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.