Thermo Fisher Scientific PD-1 Monoclonal Antibody (10B5)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 2–10 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 0.5–5 µg/10^6 cells

Product Specifications

Product Specific Information

• Positive control: Tosil
• Cellular location: Membrane

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC).
T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC.
PD-1 binds two related ligands, PDL-1 and PDL-2. Upon binding to either ligand, PD-1 generates inhibitory signals that suppress immune activation in the absence of “danger signals” such as LPS or pathogen-associated molecules.
Evidence for this is seen in PD1- mice, which exhibit hyperactivated immune systems and autoimmune diseases.
Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.