Thermo Fisher Scientific A-Raf Monoclonal Antibody (OTI2G9)
A-Raf 단백질 검출용 단클론 항체로 Western blot 및 IHC(Paraffin)에서 사용 가능. 인간 반응성, Mouse IgG2a 형식. 고순도 친화 크로마토그래피 정제, 안정한 PBS/BSA/glycerol buffer 제공. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–365 of ARAF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723815 |
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus.
The unregulated kinase activity of v-Raf has been associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest levels in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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