Thermo Fisher Scientific A-Raf Monoclonal Antibody (OTI2F8)
A-Raf 단백질을 인식하는 Mouse monoclonal 항체로 Western blot과 IHC(P) 분석에 적합. Human 시료 반응성. Affinity chromatography로 정제된 액상 형태이며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–365 of ARAF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723814 |
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all tested cell lines. It is the cellular homolog of v-Raf, the transforming gene product of the 3611 strain of murine sarcoma virus.
The unregulated kinase activity of v-Raf has been associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by a regulatory N-terminal domain. A-Raf, another member of the Raf gene family, shares substantial homology with Raf-1 within the kinase domain but less elsewhere. A-Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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