Thermo Fisher Scientific ATP5A1 Polyclonal Antibody
ATP5A1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫드 반응성 확인. 고순도 항원 친화 크로마토그래피 정제, 안정적 PBS/glycerol 저장 용액 형태.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 383–553 of Human ATP5A1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.66 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544980 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, K562, THP-1, mouse brain, rat brain
- Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (90%), Xenopus laevis (89%), Dog (95%), Pig (96%), Chicken (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP5A1 encodes a subunit of mitochondrial ATP synthase, which catalyzes ATP synthesis using the proton gradient across the inner membrane during oxidative phosphorylation.
ATP synthase consists of two linked multi-subunit complexes:
- F1 complex: soluble catalytic core (subunits α, β, γ, δ, ε) with stoichiometry 3α, 3β, and one each of the others
- Fo complex: membrane-spanning proton channel (subunits a, b, c)
This gene encodes the α subunit of the catalytic core.
Alternatively spliced transcript variants encoding different isoforms have been identified.
Pseudogenes are located on chromosomes 9, 2, and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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