Thermo Fisher Scientific SMAD1 Monoclonal Antibody (OTI1B9), TrueMAB

Thermo Fisher Scientific SMAD1 Monoclonal Antibody (OTI1B9), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The SMAD1 protein belongs to the SMAD family, homologous to the Drosophila gene mothers against decapentaplegic (Mad) and C. elegans gene Sma.
SMAD proteins act as signal transducers and transcriptional modulators, mediating multiple signaling pathways.
SMAD1 mediates bone morphogenetic protein (BMP) signals involved in cell growth, apoptosis, morphogenesis, development, and immune responses.
Upon BMP ligand binding, SMAD1 is phosphorylated by BMP receptor kinase and forms a complex with SMAD4 for transcription regulation.
It is targeted by SMAD-specific E3 ubiquitin ligases (SMURF1, SMURF2) and undergoes ubiquitination and proteasome-mediated degradation.
Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.