Thermo Fisher Scientific CD137 (4-1BB) Monoclonal Antibody (2G1)
CD137(4-1BB) 단백질을 표적으로 하는 Mouse IgG1 단클론 항체. Western blot, IHC, ICC, Flow cytometry에 적합. 인간, 마우스, 랫트 반응성. Protein A 정제, 2 mg/mL 농도, PBS/glycerol buffer. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G1 |
| Immunogen | Recombinant full-length protein of CD137 (4-1BB, UniProt ID: Q07011-1, antigen range: 1–255) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C for short term. For long term, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911721 |
Product Specific Information
- Positive Control: A431, A549, LOVO, rat brain tissue, rat hippocampus tissue, human liver cancer tissue, mouse brain tissue, human tonsil tissue, Jurkat
- Subcellular Location: Membrane, Extracellular space
Target Information
CD137 (also known as TNFRSF9 or 4-1BB) is an inducible costimulatory molecule mainly expressed on activated T cells.
Its ligand, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
CD137 signaling supports survival and clonal expansion of activated and memory T cells while suppressing myelopoiesis and dendritic cell development.
Activation of CD137 induces cytokine release that regulates peripheral monocyte survival.
Soluble forms of CD137 may serve as a negative regulatory mechanism for immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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