Thermo Fisher Scientific Phospho-EGFR (Tyr1197) Polyclonal Antibody
EGFR 단백질의 Tyr1197 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 고순도 Affinity chromatography로 정제되었습니다. 암 관련 EGFR 연구에 유용합니다.
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Thermo Fisher Scientific Phospho-EGFR (Tyr1197) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | View publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1197 (A-E-Y(p)-L-R) derived from Human EGFR |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2554162 |
Product Specific Information
- Suggested positive control for Western blot: HUVEC cells
- Suggested positive control for IHC: Human breast carcinoma
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates cell signaling in response to growth factors. Overexpression is associated with various cancers.
EGFR is encoded by the EGFR gene on chromosome 7 and belongs to the HER/ERbB family (ERbB2, ERbB3, ERbB4).
Ligand binding (e.g., EGF, TGF-α) induces receptor dimerization and autophosphorylation of tyrosine residues.
Overexpression has been observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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