Thermo Fisher Scientific Phospho-ROCK2 (Ser1366) Polyclonal Antibody
Phospho-ROCK2 (Ser1366) 폴리클로날 항체로, 인간·마우스·랫트에 반응하며 WB, IHC, ICC 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품으로, 세포 신호전달 연구에 적합. 장기 보관 시 -20°C에서 안정적 유지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding phospho Ser1366 of ROCK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2552247 |
Product Specific Information
- Recommended positive controls: HeLa, 293T, 293T transfected with wild type (WT) plasmids
- Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution; centrifuge briefly prior to opening the vial
Target Information
ROCK2 is the predominant ROCK isoform in skeletal muscle and acts downstream of Rho GTPases. It regulates cytokinesis, smooth muscle contraction, actin stress fiber and focal adhesion formation, and activation of the c-fos serum response element. ROCK2, an isozyme of ROCK1, is a target for the small GTPase Rho.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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