Thermo Fisher Scientific p28ING5 Polyclonal Antibody
Human p28 ING5 단백질을 인식하는 염소 유래 Polyclonal 항체로, Western blot, ELISA, IP에 적합합니다. Affinity chromatography로 정제되었으며, 액상 형태로 제공됩니다. -20°C 보관, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| ELISA | 1:4,000–1:16,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region near aa 125–150 of Human p28 ING5 protein (Inhibitor of growth family, member 5) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.52 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2. However, 100% homology is recorded for this protein from human and chimpanzee. Cross-reactivity with p28 ING5 protein from frog, mouse, rat, and chicken may not occur due to sequence variation as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.
Target Information
ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger motif commonly found in chromatin remodeling proteins. It can bind TP53 and EP300/p300, suggesting involvement in TP53-dependent regulatory pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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