Thermo Fisher Scientific HR23A Polyclonal Antibody
Human HR23A 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체로, WB, ELISA, IP에 사용 가능. 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| ELISA | 1:4,000–1:16,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole goat serum immunized with a synthetic peptide corresponding to an internal region near aa 115–140 of human HR23A protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity occurs against human HR23A protein. BLAST analysis shows 100% sequence homology with human and rabbit HR23A proteins. Cross-reactivity with dog, mouse, and rat HR23A proteins may occur due to minor sequence differences. Reactivity with HR23A from other species is not known.
Target Information
The HR23A gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to cytochrome P450. This oxidation/reduction system is found in steroidogenic tissues and is involved in bile acid and vitamin D synthesis. The gene is located on chromosome 11q22, with pseudogenes on chromosomes 20 and 21. The protein is expressed in various tissues but is not tissue-specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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