Thermo Fisher Scientific F4/80 Monoclonal Antibody (A10), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

A10

Immunogen

Fibroblast cell line ARH102 expressing CD97-EMR1 (EGF1-6)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Purification

Protein G

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745168

Product Specific Information

This antibody recognizes human EMR1 (EGF-like module containing, mucin-like, hormone receptor-like 1). EMR1 is a seven-span transmembrane protein which is a member of the EGF-TM7 family of receptors. The detailed structure and function of EMR1 is still unknown.

Reconstitute with 1.0 mL distilled water

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.