Thermo Fisher Scientific ADAMTS5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C K(636) N G Y Q S D A K G V K T F(649) of human ADAMTS5. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ADAMTS5,uniProtId:Q9UNA0-1,ncbiNodeId:9606,antigenRange:636-649,antigenLength:930,antigenImageFileName:PA1-1751A_ADAMTS5_Q9UNA0-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1751A_ADAMTS5_Q9UNA0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070008

Product Specific Information

PA1-1751A detects ADAMTS5 from porcine, bovine, and human samples. PA1-1751A is specific for ADAMTS5 and shows no cross-reactivity with other ADAMTS family members.

PA1-1751A has been successfully used in Western blot procedures. By Western blot, this antibody detects a major band at ~68 kDa protein representing ADAMTS5 from porcine cartilage extract. On human recombinant ADAMTS5 this antibody detects a major band at ~85 kDa. Depending on the extent of C-terminal processing, several smaller bands may also be seen using this antibody.

PA1-1751A immunogen is a synthetic peptide corresponding to residues C K(636) N G Y Q S D A K G V K T F(649) of human ADAMTS5. This polyclonal antibody has been referred to as: JSCKNG.

Target Information

ADAMTS5, also known as Implantin or Aggrecanase 2, is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin-5 motif) was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.