
Thermo Fisher Scientific TIMP1 Monoclonal Antibody (63515)
Human TIMP1 단백질에 특이적인 Mouse IgG2b 단일클론 항체로 Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 분석에 사용 가능. 교차반응 없음. Lyophilized 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 8–25 µg/mL |
| Immunocytochemistry (ICC/IF) | 3–25 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per million cells |
| ELISA | 0.1–0.4 µg/mL (Detection), 2–8 µg/mL (Capture) |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 63515 |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human TIMP-1 (Cys24–Ala207) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609828 |
Product Specific Information
- In ELISAs, this antibody does not cross-react with recombinant mouse TIMP-1 or rhTIMP-2.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that forms one-to-one complexes with target metalloproteinases such as collagenases, irreversibly inactivating them by binding to their catalytic zinc cofactor.
TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16.
It also functions as a growth factor regulating cell differentiation, migration, and cell death, and activates signaling cascades via CD63 and ITGB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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