Thermo Fisher Scientific Phospho-ULK1 (Ser131) Polyclonal Antibody
ULK1 단백질의 Ser131 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot 및 IHC(Paraffin) 실험에 적합. 인간, 생쥐, 랫트 반응성. PBS/glycerol buffer에 보관하며 -20°C에서 안정적.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ULK1 (Accession O75385), corresponding to amino acid residues around phosphorylated Ser131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816848 |
Product Specific Information
This antibody detects endogenous levels of ULK1 only when phosphorylated at Ser131.
Target Information
ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital cellular response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 regulates its localization and function. Knockdown of ULK1 inhibits autophagic response and rapamycin-induced autophagy, consistent with its role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. Both FIP200 and ATG13 are necessary for proper ULK1 localization and stability. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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