Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), Functional Grade, eBioscience

Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), Functional Grade

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Biotin
• FITC
• PE
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10), an ~18 kDa cytokine also known as Cytokine Synthesis Inhibitory Factor (CSIF). In mice, IL-10 is produced by Th2 cells, B1 cells, macrophages, and keratinocytes. It inhibits Th1 cytokine synthesis and T-cell proliferation, while acting as a costimulatory signal for mast cells and thymocytes.

Applications Reported:

• Capture of mouse IL-10 by ELISA and ELISPOT
• Intracellular staining for flow cytometry
• Immunohistochemistry (IHC)
• Neutralization of IL-10 bioactivity

Applications Tested:

• ELISPOT capture: 1–4 µg/mL
• Sandwich ELISA: JES5-16E3 as capture antibody, biotinylated JES5-2A5 (13-7102) as detection, and recombinant mouse IL-10 (14-8101) as standard
  Recommended standard curve: 4000 pg/mL – 30 pg/mL (doubling dilutions)

Storage and Handling:

• Use in a sterile environment
• Filtration: 0.2 µm post-manufacturing filtered
• Purity: >90% (SDS-PAGE)
• Endotoxin Level: <0.001 ng/µg (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

IL-10 encodes a cytokine primarily produced by monocytes and lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival and antibody production, inhibits NF-κB activity, and regulates the JAK-STAT pathway.
Knockout studies indicate IL-10 is essential for intestinal immune regulation. Mutations are associated with susceptibility to HIV-1 infection and rheumatoid arthritis.
Diseases related to IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.