Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 12 publications 12 publications

ELISA (ELISA)

Assay-Dependent

View 6 publications 6 publications

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Inhibition Assays (IA)

-

View 2 publications 2 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Unconjugated
• Alexa Fluor 488
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866232

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: The JES3-9D7 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The JES3-9D7 antibody has been tested for neutralizing IL-10 binding and biological activity.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.