Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), Functional Grade, eBioscience

Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

Description:
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported:
Reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested:
Tested for neutralizing IL-10 binding and biological activity.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody, determined by LAL assay.

Aggregation:
<10%, determined by HPLC.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It plays a key role in immunoregulation and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, and can block NF-kappa B activity. It is involved in the JAK-STAT signaling pathway. Mouse knockout studies indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis. Associated diseases include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.