Thermo Fisher Scientific RNF185 Polyclonal Antibody
RNF185 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot에 최적화되어 있으며, 인간 RNF185 중심부 서열 기반 항원으로 제작됨. 항원 친화 크로마토그래피로 정제, 4°C 단기 보관 또는 -20°C 장기 보관 권장. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RNF185 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.68 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736531 |
Product Specific Information
- Positive Control: Human RNF185-transfected 293T
- Predicted Reactivity: Mouse (98%), Rat (98%), Zebrafish (83%), Xenopus laevis (91%), Chicken (87%), Bovine (98%)
- Usage Note: Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RNF185 is an E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. It functions in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, targeting misfolded proteins in the ER for ubiquitination and subsequent proteasome-mediated degradation. RNF185 protects cells from ER stress-induced apoptosis and is involved in cotranslational ubiquitination and degradation of CFTR, preferentially associating with E2 enzymes UBE2J1 and UBE2J2.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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