Thermo Fisher Scientific RNF185 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF185. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.68 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2736531

Product Specific Information

Positive Control: human RNF185-transfected 293T

Predicted Reactivity: Mouse (98%), Rat (98%), Zebrafish (83%), Xenopus laevis (91%), Chicken (87%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RNF185 is a E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. RNF185 acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. RNF185 protects cells from ER stress-induced apoptosis. is responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway, and is preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.