Thermo Fisher Scientific SPARC Monoclonal Antibody (N50)
SPARC 단백질을 타깃으로 하는 Thermo Fisher Scientific의 단클론 항체(N50). Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. 마우스 IgG1 형식으로 높은 친화도(5×10⁹ M)를 가짐. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Radioimmune Assays (RIA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N50 |
| Immunogen | Tissue / cell preparation (crude bone extract containing native osteonectin) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 7.3 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2255293 |
Product Specific Information
Affinity constant: 5 × 10⁹ M
Target Information
The SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified and is involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but also correlated with metastasis due to its role in promoting tumor cell invasion. Three transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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