
Thermo Fisher Scientific ERp57 Monoclonal Antibody (Map.ERp57), APC
ERp57 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체로, APC 형광 표지로 다양한 면역분석(WB, IHC, ICC, IP)에 사용 가능. 인간 및 여러 종에 반응하며, 고순도 Protein G 정제 및 4°C 보관 조건을 가짐.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Map.ERp57 |
| Immunogen | Human recombinant ERp57 (Grp58) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931681 |
Additional Formats
- FITC (Cat. MA5-45228)
- PerCP (Cat. MA5-45229)
- Custom conjugation available upon request
Product Specific Information
0.5 µg/mL of MA5-45227 was sufficient for detection of ERp57 in 10 µg of heat shock HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 57 kDa.
Target Information
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein has protein disulfide isomerase activity and assists in disulfide bond formation in glycoprotein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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