Thermo Fisher Scientific RIP3 Polyclonal Antibody
RIP3 단백항체로 인간 RIPK3 단백질을 검출하는 데 사용. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 고순도 항원 친화 크로마토그래피로 정제된 rabbit polyclonal IgG. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific RIP3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RIP3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.56 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792827 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: THP-1.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm and specifically colocalized in the mitochondrion, which can undergo nucleocytoplasmic shuttling dependent on nuclear localization and export signals. RIPK3 is a component of the tumor necrosis factor (TNF) receptor-I signaling complex and can induce apoptosis and weakly activate the NF-kappaB transcription factor. Caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR, which is required for the protective function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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