Thermo Fisher Scientific OBFC2B Monoclonal Antibody (OTI1E11), TrueMAB
OBFC2B 단백질을 인식하는 Mouse Monoclonal Antibody로, Western blot, IHC, Flow Cytometry에 적합합니다. 고순도의 Affinity chromatography 정제 제품이며, Lyophilized 형태로 제공됩니다. DNA 손상 반응 및 복구 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E11 |
| Immunogen | Full length human recombinant protein of human OBFC2B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
OBFC2B is a component of the SOSS complex, which acts downstream of the MRN complex to promote DNA repair and G2/M checkpoint control.
Within the SOSS complex, OBFC2B functions as a sensor of single-stranded DNA, particularly binding to polypyrimidines.
The SOSS complex associates with DNA lesions and contributes to cell-cycle checkpoint activation, recombinational repair, and genomic stability maintenance.
It is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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