Thermo Fisher Scientific Ethenoadenosine Monoclonal Antibody (1G4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:800

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Flow Cytometry (Flow)

1:10-1:1,000

-

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG2, lambda

Class

Monoclonal

Type

Antibody

Clone

1G4

Immunogen

Ethenoadenosine.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568528

Target Information

The reaction of chloroacetaldehyde (CAA) with DNA results in the formation of ethenoadenosine also known as 1,N6-ethenodeoxy-adenosine or etheno-A. CAA is formed through metabolization of for example vinyl chloride, a well established carcinogen. In RNA, after in vitro activation, etheno-A and etheno-C are the principle products of RNA damage. Etheno derivate formation may be highest in single-stranded DNA-regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.