Thermo Fisher Scientific ATGL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 45-257 of human PNPLA2 (NP_0651091) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATGL,uniProtId:Q96AD5-1,ncbiNodeId:9606,antigenRange:45-257,antigenLength:504,antigenImageFileName:PA5-110189_ATGL_Q96AD5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-110189_ATGL_Q96AD5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.24 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855600

Product Specific Information

Immunogen sequence: GASAGALTAT ALVTGVCLGE AGAKFIEVSK EARKRFLGPL HPSFNLVKII RSFLLKVLPA DSHEHASGRL GISLTRVSDG ENVIISHFNS KDELIQANVC SGFIPVYCGL IPPSLQGVRY VDGGISDNLP LYELKNTITV SPFSGESDIC PQDSSTNIHE LRVTNTSIQF NLRNLYRLSK ALFPPEPLVL REMCKQGYRD GLRFLQRNGL LNR

Target Information

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.