Thermo Fisher Scientific PD-L1 Monoclonal Antibody (OTI9E10), TrueMAB
PD-L1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot과 유세포분석에 사용 가능. 인간 유래 PD-L1(AA 19-290) 항원을 면역원으로 제작. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. 종양 면역 회피 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9E10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 19–290 of human CD274 (PD-L1) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Programmed death receptor ligand 1 (PD-L1, also known as B7-H1) is a member of the B7 family.
It binds to its receptor PD-1, which negatively regulates T-cell receptor (TCR) signaling, leading to reduced T-cell proliferation and cytokine production.
PD-L1 expression is abundant in many murine and human cancers and can be upregulated by IFN-γ stimulation, suggesting its key role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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