Thermo Fisher Scientific Human NT-3 Recombinant Protein
인간 유래 NT-3 재조합 단백질로 신경세포 생존 및 분화를 조절하는 연구용 시약입니다. E. coli에서 발현되었으며 ≥98% 순도, 낮은 엔도톡신 수준(<0.1 ng/µg)을 보장합니다. 기능적 활성 검증 완료, 다양한 생화학 및 세포 기능 분석에 적합합니다.
- 카탈로그번호
- PHC7036
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific Human NT-3 Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 20–50 ng/mL; determined by dose-dependent induction of choline acetyl transferase activity in rat basal forebrain septal cultures |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
- Briefly centrifuge vial before opening to bring contents to the bottom.
- Reconstitute NT-3 in water to a concentration of 0.1–1.0 mg/mL.
- Do not vortex.
- Store reconstituted solution at 2°C–8°C for up to 1 week.
- For extended storage, dilute in buffer containing a carrier protein (e.g., 0.1% BSA) and store aliquots at -20°C to -80°C.
Storage:
- Lyophilized protein stable at room temperature for 1 month.
- For long-term storage, keep at -20°C.
- Working aliquots with carrier protein are stable for ≥3 months at -20°C to -80°C.
- Avoid repeated freeze/thaw cycles.
Target Information
The protein encoded by this gene is a member of the neurotrophin family, which controls survival and differentiation of mammalian neurons. It is closely related to nerve growth factor and brain-derived neurotrophic factor. This protein may be involved in maintenance of the adult nervous system and neuronal development in embryos when expressed in human placenta.
NTF3-deficient mice show severe limb movement defects. The mature peptide is identical across mammals including human, pig, rat, and mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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