Thermo Fisher Scientific Human NT-4 Recombinant Protein
인간 유래 NT-4 재조합 단백질로, 신경영양 인자 연구에 적합합니다. E. coli 발현 시스템에서 생산되었으며 ≥98% 순도를 보장합니다. 다양한 어세이(WB, IHC, ELISA 등)에 활용 가능하며, 안정적인 생물학적 활성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
| Functional Assay | Assay-dependent | - |
| Control (Ctrl) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human NT-4 recombinant protein is a homodimer containing two 130 amino acid subunits |
| Molecular Weight | 27.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 20–50 ng/mL; determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain septal cultures |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
Briefly centrifuge vial before opening. Reconstitute rhNT-4 in sterile distilled water at 0.1–1.0 mg/mL. Do not vortex.
Store reconstituted solution at 2–8°C for up to 1 week.
For long-term storage, dilute with buffer containing 0.1% BSA and store aliquots at -20°C to -80°C.Storage:
Lyophilized protein is stable at room temperature for 1 month.
For long-term storage, keep at -20°C.
Working aliquots with carrier protein are stable for at least 3 months at -20°C to -80°C.
Avoid repeated freeze/thaw cycles.
Target Information
This gene belongs to the neurotrophin family, which regulates survival and differentiation of mammalian neurons.
Unlike other neurotrophins (NGF, BDNF, NT-3), NTF5-deficient mice show minor cellular deficits and develop normally to adulthood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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