Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (16B1), APC-eFluor 780, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Biotin
• PE
• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

Description:
The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. This calcium-dependent adhesion molecule is localized at intercellular boundaries of endothelial cells in blood and lymphatic vessels, playing a role in vascular permeability and remodeling.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). Recommended usage: 5 µL (0.25 µg) per test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633–647 nm). Ensure instrument compatibility with this fluorochrome.
Light-sensitive: protect from light to avoid photo-induced oxidation.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-57)
  for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser

Target Information

VE-cadherin is a member of the cadherin superfamily located in a six-cadherin cluster on chromosome 16. It functions as a calcium-dependent cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It plays an important role in endothelial cell cohesion and intercellular junction organization. An alternative splice variant has been described, though the full-length sequence has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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