Thermo Fisher Scientific c-Fos Monoclonal Antibody (T.142.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:6,400

View 1 publication 1 publication

Flow Cytometry (Flow)

1:1,600-1:6,400

-

ChIP assay (ChIP)

1:50

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

T.142.5

Immunogen

Synthetic peptide corresponding to the sequence of human c-Fos

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

108 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10984728

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other Fos proteins, including FosB, FRA1 and FRA2.

Target Information

FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.