Thermo Fisher Scientific c-Fos Monoclonal Antibody (T.142.5)
c-Fos 단백질을 특이적으로 인식하는 Rabbit Monoclonal Antibody. Western blot, IHC, ICC/IF, Flow, ChIP 등 다양한 응용 가능. 다른 Fos 단백질과 교차 반응 없음. 고순도 친화 크로마토그래피 정제 제품으로 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 3 publications |
| Immunohistochemistry (IHC) | – | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:6,400 | 1 publication |
| Flow Cytometry (Flow) | 1:1,600–1:6,400 | – |
| ChIP assay (ChIP) | 1:50 | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | T.142.5 |
| Immunogen | Synthetic peptide corresponding to the sequence of human c-Fos |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 108 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10984728 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other Fos proteins, including FosB, FRA1, and FRA2.
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of multiple genes involved in cellular signal transduction, proliferation, and differentiation.
It is phosphorylated by various stimuli including growth factors and insulin at several sites. The best-known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK, which regulates nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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