Thermo Fisher Scientific alpha Actinin 3 Recombinant Rabbit Monoclonal Antibody (4H7E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4H7E6

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 700-800 of human ACTN3 (Q08043). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Actinin 3,uniProtId:Q08043-1,ncbiNodeId:9606,antigenRange:700-800,antigenLength:901,antigenImageFileName:MA5-42718_alpha_Actinin_3_Q08043-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42718_alpha_Actinin_3_Q08043-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911859

Product Specific Information

Positive test controls include: Mouse skeletal muscle, Rat skeletal muscle.

Immunogen sequence: DRLEGDHQLL QESLVFDNKH TVYSMEHIRV GWEQLLTSIA RTINEVENQV LTRDAKGLSQ EQLNEFRASF NHFDRKQNGM MEPDDFRACL ISMGYDLGEV E

Target Information

Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.