
Thermo Fisher Scientific Rabbit anti-Human IgG (Heavy chain) Recombinant Secondary Antibody, Biotin
Human IgG(Heavy chain)에 특이적인 Biotin 결합형 Rabbit Recombinant Monoclonal Secondary Antibody. IHC, ICC/IF, ELISA에 적합. 높은 특이성과 일관된 로트 간 품질 제공. Protein A 정제, PBS/BSA/glycerol buffer로 안정적 보관 가능.
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Applications 및 권장 희석 농도
| 응용 분야 | 권장 희석 농도 |
|---|---|
| Immunohistochemistry (IHC) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.05–1 µg/mL |
제품 사양
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM116 |
| Immunogen | Human IgG |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744970 |
| Target | IgG (Heavy chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to the larger rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified and characterized for specificity to human immunoglobulins. They are useful for detection, sorting, or purification of target antibodies.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, hosts are immunized with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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