Thermo Fisher Scientific H3.3 K27M oncohistone mutant Recombinant Rabbit Monoclonal Antibody (RM192), ChIP-Verified
H3.3 K27M 변이 온코히스톤에 특이적인 재조합 토끼 단클론 항체로, ChIP 검증 완료. Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용에 사용 가능. 높은 특이성과 재현성, 동물 유래 성분 무첨가 포뮬레이션 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250–1:2500 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:250–1:2500 |
| ChIP assay (ChIP) | 1–5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM192 |
| Immunogen | A peptide corresponding to Histone H3 K27M mutant |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744969 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Click here for Master Lot linking information
Target Information
Histone H3 is a DNA-binding protein found in eukaryotic chromatin. Together with other core histones, it forms the nucleosome structure. Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes various post-translational modifications—such as methylation, acetylation, and phosphorylation—that affect chromatin structure and gene expression by influencing transcription factor binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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