Thermo Fisher Scientific H3.3 K27M oncohistone mutant Recombinant Rabbit Monoclonal Antibody (RM192), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:2500

-

Immunohistochemistry (IHC)

1:100-1:500

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:500

-

ELISA (ELISA)

1:250-1:2,500

-

ChIP assay (ChIP)

1-5 µg

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM192

Immunogen

A peptide corresponding to Histone H3 K27M mutant if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3.3K27M,uniProtId:P84243-1,ncbiNodeId:9606,antigenRange:27,antigenLength:136,antigenImageFileName:MA5-27916_H3.3K27M_P84243-1_Rabbit.svg,antigenImageFileNamePDP:MA5-27916_H3.3K27M_P84243-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

0.25 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2744969

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Click here for Master Lot linking information.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.