Thermo Fisher Scientific HA Tag Recombinant Rabbit Monoclonal Antibody (RM305)
HA 태그 단백질 검출용 재조합 토끼 단클론 항체. Western blot, IHC, ICC, Flow cytometry, IP, ChIP 등 다양한 응용에 사용 가능. 높은 특이성과 재현성 제공. 단백질 정제 및 위치 분석 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–1 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | 0.01–0.5 µg/mL | – |
| Immunocytochemistry (ICC/IF) | 0.1–1 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 0.1–1 µg/mL | – |
| Immunoprecipitation (IP) | 0.5–2 µg/mL | View 1 publication |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM305 |
| Immunogen | A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98–106) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744968 |
Additional Formats:
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
HA Tag (hemagglutinin tag) is an epitope tag derived from influenza hemagglutinin. It is used to:
- Localize gene products in various cell types
- Study protein topology and complexes
- Identify associated proteins
- Characterize low abundance or poorly immunogenic proteins when specific antibodies are unavailable
HA tag antibodies facilitate detection and co-immunoprecipitation of tagged proteins for functional analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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